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Primary Antibodies
FKBP15 Antibody
Background
FKBP15, also known as FKBP133, is a member of the FK506-binding protein family, a group of proteins initially identified as immunophilins, targets for the immunosupressant drugs FK506 and Rapamycin. FKBP15 is expressed in the developing nervous system and contains a domain similar to Wiskott-Aldrich syndrome protein homology region 1 (WH1) in addition to the FK506-binding protein motif. FKBP15 is distributed along the axonal shafts and partially co-localizes with F-actin in the growth cones of dorsal root ganglion neurons; overexpression of FKBP15 resulted in the number of filopodia in transfected neurons, suggesting that FKBP15 modulates growth cone behavior. FKBP15 has also been shown to associate with both microtubules and the actin filament systems and disruption of its expression by RNAi resulted in delayed transport of early endosomes in HeLa cells indicating that FKBP15 is also involved in the transport of early endosomes. At least three isoforms of FKBP15 are known to exist.
Additional Names
FKBP15, FK506-binding protein homolog, FKBP133, WASP and FKBP-like, WAFL
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Description
Left: Western blot analysis of FKBP15 in 3T3 cell lysate with FKBP15 antibody at 1 μg/ml.
Below: Immunohistochemistry of FKBP15 in mouse brain tissue with FKBP15 antibody at 2.5 μg/ml.
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Other Product Images
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Source
FKBP15 antibody was raised against a 15 amino acid peptide from near the amino terminus of human FKBP15.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
FKBP15 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
FKBP15 antibody can be used for detection of FKBP15 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5135P - FKBP15 Peptide
Storage
FKBP15 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
150010552
Protein Accession Number
NP_056073
Short Description
FK506-binding protein homolog
This product belongs to the following categories:
Related Products
References- Nakajima O, Nakamura F, Yamashita N, et al. FKBP133: A novel mouse FK506-binding protein homolog alters growth cone morphology. Biochem. Biophys. Res. Comm. 2006; 346:140-9.
- Snyder SH, Lai MM, and Burnett PE. Immunophilins in the nervous system. Neuron 1998; 21:283-94.
- Viklund I-M, Aspenstrom P, Meas-Yedid V, et al. WAFL, a new protein involved in regulation of early endocytic transport at the intersection of actin and microtubule dynamics. Exp. Cell Res. 2009; 315:1040-52.
Datasheet 09-01W
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