|
Primary Antibodies
FGA Antibody
Background
FGA is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in its gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis.The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus.
Additional Names
FGA, Fib2
|
Description
Left: Western blot analysis of FGA in Jurkat Cell Lysate using FGA antibody at 5 μg/ml (12% gel). Marker in lane A.
|
Purification
Antibody is purified by protein A chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
FGA antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FGA.
Application
FGA antibody can be used for detection of FGA by ELISA at 1:1562500. FGA antibody can be used for detection of FGA by western blot at 5.0 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB, IHC
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store FGA antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H
Protein Accession Number
NP_068657
Molecular Weight
71kDa, 95kDa
Short Description
FGA (fibrinogen alpha chain)
This product belongs to the following categories:
References- Smolarczyk,K., (2005) Arterioscler. Thromb. Vasc. Biol. 25 (12), 2679-2684.
Datasheet 10-01W
|
|
