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Primary Antibodies
FGA Antibody
Background
FGA is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in its gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis.The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus.
Additional Names
FGA, fibrinogen alpha chain
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Description
Left: Western blot analysis of FGA expression in jurkat cell lysate using FGA antibody 5.0µg/ml. Lane A is a marker, and the lysate is in lane B.
Below: Immunocytochemistry of human skin tissue using FGA antibody at 4.0 - 8.0µg/ml.
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Purification
Rabbit IgG purified by protein A chromatography.
Clonality / Clone
This is a polyclonal antibody.
Host
FGA antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human FGA.
Application
FGA antibody can be used for Western blot at a suggested dilution at 5.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Tested Application
WB, IHC
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 ?l of distilled water. Final antibody concentration is 2 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_068657
Molecular Weight
71kDa , 95kDa
Short Description
Anti-FGA
This product belongs to the following categories:
Related Products
References- Smolarczyk,K., (2005) Arterioscler. Thromb. Vasc. Biol. 25 (12), 2679-2684
Datasheet 0802-W
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