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Primary Antibodies
FEZ2 Antibody
Background
Fasciculation and elongation protein zeta-2 (FEZ2) is a homolog to the mammalian FEZ1, itself an ortholog of the C. elegans UNC-76. In contrast to FEZ1, FEZ2 mRNA is widely expressed in mouse tissues. FEZ2 interacts with protein kinase C (PKC)-z substrate and induces neurite extension of PC12 cells when co-expressed with a constitutively active form of PKC-z, suggesting FEZ2 may play an important role in the morphological changes of various cells by associating with PKC-z in a tissue non-specific manner. FEZ2 can interact with FEZ1 through its c-terminal regions and especially its coiled-coil region. At least two isoforms of FEZ2 are known to exist.
Additional Names
FEZ2 (IN), Fasciculation and elongation protein zeta-2, zygin 2
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Description
Left: Western blot analysis of FEZ2 in 3T3 cell lysate with FEZ2 antibody at (A) 0.5, and (B) 1 μg/ml.
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Source
FEZ2 antibody was raised against a 15 amino acid peptide from near the center of human FEZ2.
Clonality / Clone
This is a polyclonal antibody.
Host
FEZ2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
FEZ2 antibody can be used for the detection of FEZ2 by Western blot at 0.5 – 1 μg/ml.
Tested Application
WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
FEZ2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1212 - 3T3 Cell Lysate
Species Reactivity
H,M,R
Protein Accession Number
NP_001036013
Data Sheet Version
08-02W
Related Products
References
- Fujita T, Ikuta J, Okajima T, et al. Identification of a tissue-non-specific homologue of axonal fasciculation and elongation protein zeta-1. Biochem. Biophys. Res. Commun. 2004; 313:738-44.
- Kuroda S, Nakagawa N, Tokunaga C, et al. Mammalian homologue of the Caenorhabditis elegans UNC-76 protein involved in axonal outgrowth is a protein kinase C z-interacting protein. J. Cell Biol. 1999; 144:403-11.
- Assmann EM, Alborghetti MR, Camargo MER, et al. FEZ1 dimerization and interaction with transcription regulatory proteins involves its coiled-coil region. J. Biol. Chem. 2006; 281:9869-81.
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