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Primary Antibodies
FAIM Antibody
Background
The susceptibility of primary splenic B cells to Fas-mediated apoptosis is regulated in a receptor-specific fashion. Terminal effectors of B cell Fas-resistance include the known anti-apoptotic proteins Bcl-xL, FLIP, and a recently identified protein termed FAIM. This molecule is broadly expressed in various tissues and exists in at least three isoforms. It is thought that resistance to Fas killing via increased expression of FAIM protects foreign antigen-specific B cells during interactions with FasL-bearing T cells whereas autoreactive B cells are deleted via Fas-dependent cytotoxicity. More recent results have indicated that FAIM interacts with both Trk and p75 neurotrophin receptor and may play a role in promoting neurite outgrowth in different neuronal systems by a mechanism involving the activation of NF-κB and the Ras-ERK pathway.
Additional Names
FAIM, Fas apoptotic inhibitory molecule
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Description
Left: Western blot analysis of FAIM in human spleen tissue lysate with FAIM antibody at (A) 5 and (B) 10 μg/ml.
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Source
FAIM antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human FAIM .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
FAIM antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
FAIM antibody can be used for detection of FAIM by Western blot at 5 – 10 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 2309P - FAIM Peptide
Storage
FAIM antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity
H, M
Protein GI Number
8922536
Protein Accession Number
NP_060617
Short Description
an apoptosis inhibitor
This product belongs to the following categories:
Related Products
References- Rothstein TL. Inducible resistance to Fas-mediated apoptosis in B cells. Cell Res. 2000; 10:245-66.
- Schneider TJ, Fischer GM, Donohoe TJ, et al. A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes. J. Exp. Med. 1999; 189:949-55.
- Sole C, Dolcet X, Segura MF, et al. The death receptor antagonist FAIM promotes neurite outgrowth by a mechanism that depends on ERK and NF-kappa B signaling. J. Cell Biol. 2004; 167:479-92.
Datasheet 08-02W
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