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Primary Antibodies
FAF1 Antibody
Background
Fas-associated protein 1 (FAF1) was initially identified as a Fas-binding pro-apoptotic protein that is component of the death-inducing signaling complex in Fas-mediated apoptosis (1). FAF1 can also induce apoptosis in the absence of extrinsic death signals when overexpressed although it does not contain typical death motifs such as the death domain, death effector domain, and caspase recruitment domain (1,2). Overexpression of FAF1 also decreases the basal level of NF-κB activity in transfected 293 cells, inhibits NF-κB activity induced by TNF-α, IL-1β and lipopolysaccharide, and prevents NF-κB translocation to the nucleus (3), suggesting that another role of FAF1 is to negatively regulate the activity of NF-κB. FAF1 can also interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway (4). Multiple differentially spliced isoforms of FAF1 are known to exist.
Additional Names
FAF1 (CT), Fas-associated protein 1, hFAF1
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Description
Left: Western blot analysis of FAF1 in Jurkat cell lysate with FAF1 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of FAF1 in rat spleen tissue with FAF1 antibody at 2.5 μg/ml.
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Other Product Images
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Source
FAF1 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human FAF1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
FAF1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
FAF1 antibody can be used for detection of FAF1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3993P - FAF1 Peptide
Storage
FAF1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
- Cat. No. 1466 - Rat Spleen Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
5901948
Protein Accession Number
NP_008982
Short Description
(CT) Fas-associated protein 1
This product belongs to the following categories:
Related Products
References- Chu K, Niu X, and Williams LT. A Fas-associated protein factor, FAF1, potentiates Fas-mediated apoptosis. Proc. Natl. Acad. Sci. USA 1995; 92:11894-8.
- Ryu SW and Kim E. Apoptosis induced by human Fas-associated factor 1, hFAF1, requires its ubiquitin homologous domain, but not the Fas-binding domain. Biochem. Biophys. Res. Commun. 2001; 286:1027-32.
- Park M-Y, Jang HD, Lee SY, et al. Fas-associated Factor-1 inhibits Nuclear Factor-κB (NF-κB) activity by interfering with nuclear translocation of the RelA (p65) subunit of NF-κB. J. Biol. Chem. 2004; 279:2544-9.
- Kinoshita T, Kondoh C, Hasegawa M, et al. Fas-associated factor 1 is a negative regulator of PYRIN-containing Apaf-1-like protein 1. Int. Immunol. 2006;18:1701-6.
Datasheet 08-02W
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