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Primary Antibodies
Emerin Antibody
Background
Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family that includes proteins such as LAP2 and MAN1. Each family member, including Emerin, has an ~40 amino acid LEM-domains that binds barrier-to-autointegration (BANF1), a conserved chromatin protein that also serves as a host cell component of retroviral integration complexes, including that of HIV. Emerin is anchored at the inner membrane of the nuclear envelope where it binds to nuclear intermediate filaments that are formed by lamin proteins. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
Additional Names
Emerin, Emery-Dreifuss muscular dystrophy, EDMD, EMD
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Description
Left: Western blot analysis of Emerin in human skeletal muscle tissue lysate with Emerin antibody at (A) 0.5 and (B) 1 μg/ml.
Below: Immunohistochemistry of emerin in human skeletal muscle tissue with emerin antibody at 2.5 μg/ml.
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Other Product Images
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Source
Emerin antibody was raised against a 19 amino acid peptide from near the amino terminus of human Emerin.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Emerin antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Emerin antibody can be used for detection of Emerin by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4031P - Emerin Peptide
Storage
Emerin antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
4557553
Protein Accession Number
NP_000108
Short Description
a serine-rich nuclear membrane member of the nuclear lamina-associated protein family
This product belongs to the following categories:
Related Products
References- Schirmer EC, Florens L, Guan T, et al. Nuclear membrane proteins with potential disease links found by subtractive proteomics. Science 2003; 301:1380-2.
- Cai M, Huang Y, Ghirlando R, et al. Solution structure of the constant region of nuclear envelope protein LAP2 reveals two LEM-domain structures: one binds BAF and the other binds DNA. EMBO J. 2001; 20:4399-407.
- Chen H and Engelman A. The barrier-to-autointegration protein is a host factor for HIV type 1 integration. Proc. Natl. Acad. Sci. USA 1998; 95:15270-4.
- Hutchison CJ. Lamins: building blocks or regulators of gene expression? Nat. Rev. Mol. Cell Biol. 2002; 3:848-58.
Datasheet 08-02W
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