|
Lysates
Ear Lysate
Background
Rabbit ear tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The rabbit ear tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the ear tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The ear tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Tissue Type
Ear
Diagnosis
Normal
Source
Rabbit ear tissue
Application
Ear lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Tested Application
WB
Storage
Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Species Reactivity
n/a
Species
Rabbit
Short Description
total protein ear - rabbit
This product belongs to the following categories:
Datasheet 0704W
|
|
