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27-526


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GeneDireX Molecular Weight Marker


Antibody Production


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Primary Antibodies

EGR1 Antibody

Background

Early Growth Response Protein 1 (EGR1, Krox-24 protein, nerve growth factor-induced protein A, Transcription factor ETR103, Zinc finger protein 225) belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Additional Names

EGR1, AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225

EGR1 Antibody

Description

Left: Western blot analysis of EGR1 in Fetal Lung Lysate using EGR1 antibody at 1 μg/ml (12% gel).

Purification

Antibody is purified by peptide affinity chromatography method.

Clonality / Clone

This is a polyclonal antibody.

Host

EGR1 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGR1.

Application

EGR1 antibody can be used for detection of EGR1 by ELISA at 1:62500. EGR1 antibody can be used for detection of EGR1 by western blot at 1 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Tested Application

E, WB

Buffer

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.

Storage

For longer periods of storage, store EGR1 antibody at -20˚C. Avoid repeat freeze-thaw cycles.

Species Reactivity

H

Protein Accession Number

NP_001955

Molecular Weight

57kDa

Short Description

EGR1 (early growth response 1)

This product belongs to the following categories:

References

  1. Akutagawa,O., (2008) Cancer Sci. 99 (7), 1401-1406.


Datasheet 10-01W

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