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Primary Antibodies
EGR1 Antibody
Background
Early Growth Response Protein 1 (EGR1, Krox-24 protein, nerve growth factor-induced protein A, Transcription factor ETR103, Zinc finger protein 225) belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.
Additional Names
AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225
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Description
Left: Western blot analysis of EGR1 in Jurkat cell lysate using anti-EGR1.
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Purification
Rabbit IgG is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
EGR1 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
EGR1 antibody was produced in rabbits immunized with a synthetic peptide corresponding to a region of human EGR1.
Application
EGR1 antibody can be used for Western blot at a suggested dilution of 1.0-5.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1: 50,000 - 100,037 as secondary antibody. The protein size is 543 amino acids.
Tested Application
WB, IHC
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
EGR1 antibody is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ºC. Avoid repeat freeze-thaw cycle.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_001955
Molecular Weight
58kDa
Short Description
Anti-Early Growth Response Protein 1 (EGR1)
This product belongs to the following categories:
Related Products
References- Yu,J.,et al.,(2004) Mol.Cell 15(1),83-94
Datasheet 0802-W
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