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Primary Antibodies
EEA1 Antibody
Background
EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25.
Additional Names
EEA1, early endosome antigen 1
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Description
Left: Western blot analysis of EEA1 expression in placenta tissue lysate using EEA1 antibody 0.06µg/ml.
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Purification
Rabbit Ig G is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
EEA1 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human EEA1.
Application
EEA1 antibody can be used for Western blot at a suggested dilution at 0.06µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Tested Application
WB
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Positive Control
- Cat. No. 1309 - Human Placenta Lysate
Species Reactivity
H
Protein Accession Number
NP_003557
Molecular Weight
162kDa
Short Description
Anti-EEA1
This product belongs to the following categories:
Related Products
References- Fratti,R.A., et al., (2003) J. Biol. Chem. 278 (47), 46961-46967
Datasheet 0802-W
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