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Primary Antibodies
ECSIT Antibody
Background
Activation of NF-?B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.
Additional Names
ECSIT (CT), ECSIT
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Description
Left: Western blot analysis of ECSIT in human heart cell lysates with ECSIT antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunohistochemistry of ECSIT in mouse heart cells with ECSIT antibody at 2 μg/ml.
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Other Product Images
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Source
ECSIT antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human ECSIT.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
ECSIT antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human ECSIT (C-Terminus) Peptide (Cat. No. 3377P)
Application
ECSIT antibody can be used for detection of ECSIT by Western blot at 0.5 to 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3377P - ECSIT Peptide
Storage
ECSIT antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1301 - Human Heart Tissue Lysate
- Cat. No. 1401 - Mouse Heart Tissue Lysate
Species Reactivity
H, M
Protein GI Number
20149633
Protein Accession Number
NP_057665
Short Description
(CT) An intermediate in the TLR and Bmp signaling pathways
This product belongs to the following categories:
Related Products
References- Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
- Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
- Janssens S and Beyaert R. Functional diversity and regulation of different interleukin-1 receptor-associated kinase (IRAK) family members. Mol. Cell. 2003; 11:293-302.
- Sato S, Sugiyama M, Yamamoto M, et al. Toll/IL-1 receptor domain-containing adaptor inducing IFN-β (TRIF) associates with TNF receptor-associated factor 6 and TANK-binding kinase 1, and activates two distinct transcription factors, NF-κ B and IFN-regulatory factor-3, in the Toll-like receptor signaling. J. Immunol. 2003; 171(8):4304-10.
Datasheet 08-02W
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