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Primary Antibodies
EBI3 Antibody
Background
EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35 (1-3). Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-g, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.
Additional Names
EBI3 (IN), Epstein-Barr virus induced 3 precursor, interleukin-27 subunit beta, IL-27B
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Description
Left: Western blot analysis of EBI3 in rat spleen tissue lysate with EBI3 antibody at 2 μg/ml.
Below: Immunohistochemistry of EBI3 in human spleen tissue with EBI3 antibody at 2.5 μg/ml.
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Other Product Images
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Source
EBI3 antibody was raised against a 16 amino acid peptide from near the center of human EBI3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
EBI3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
EBI3 antibody can be used for the detection of EBI3 by Western blot at 2 – 4 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4977P - EBI3 Peptide
Storage
EBI3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1466 - Rat Spleen Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
14577917
Protein Accession Number
NP_005746
Short Description
(IN) Epstein-Barr virus induced 3 precursor
This product belongs to the following categories:
Related Products
References- Pfanz S, Timans JC, Cheung J et al. IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naïve CD4(+) T cells. Immunity 2002; 16:779-90.
- Devergne O, Birkenbach M, and Kieff E. Epstein-Barr virus-induced gene 3 and the p35 subunit of interleukin form a novel heterodimeric hematopoietin. Proc. Natl. Acad. Sci. USA 1997; 94:12041-6.
- Niedbala W, Wei X, Cai B, et al. IL-35 is a novel cytokine with therapeutic effects against collagen-induced arthritis through the expression of regulatory T cells and suppression of Th17 cells. Eur. J. Immunol. 2007; 3021-29.
- Pfanz S, Hibbert L, Mattson J, et al. WSX-1 and glycoprotein 130 constitute a signal-transducing receptor for IL-27. J. Immunol. 2004; 172:2225-31.
Datasheet 09-01W
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