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Primary Antibodies
E2F4 Antibody
Background
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It also plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer **Cat.No. XAV-8476
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Additional Names
E2F-4
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Description
Left: Western blot analysis of E2F4 in HepG2 cell lysate using anti-E2F4.
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Purification
Rabbit IgG is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
E2F4 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
E2F4 antibody was produced in rabbits immunized with a synthetic peptide corresponding to a region of human E2F4.
Application
E2F4 antibody can be used for Western blot at a suggested dilution of 1.0-5.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. The protein size is 413 amino acids.
Tested Application
WB, IHC
Buffer
Antibody is supplied in PBS buffer with 2% sucrose.
Storage
E2F4 antibody is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ºC. Avoid repeat freeze-thaw cycle.
Positive Control
- Cat. No. 1211 - HepG2 Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_001941
Molecular Weight
44kDa
This product belongs to the following categories:
Related Products
References- DuPree,E.L., et al. (2004) Cancer Res. 64 (13), 4390-4393
Datasheet 0802-W
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