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Primary Antibodies
E2F1 Antibody
Background
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
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Description
Left: Western blot analysis of E2F1 expression in Jurkat (A), HepG2 (B), and Raji (C) cell lysates using anti-E2F1.
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Purification
Rabbit IgG is purified by Protein A/G affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
E2F1 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
E2F1 antibody was produced in rabbits immunized with a synthetic peptide corresponding to a region of human E2F1.
Application
E2F1 antibody can be used for Western blot at a suggested dilution of 1 μg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. The protein size is 437 amino acids.
Tested Application
WB
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
E2F1 antibody is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ºC. Avoid repeat freeze-thaw cycle.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
- Cat. No. 1211 - HepG2 Cell Lysate
- Cat. No. 1207 - Raji Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_005216
Molecular Weight
47 kDa
Short Description
Anti-E2F1
This product belongs to the following categories:
Related Products
References- Araki, K., et al., (2003) Oncogene 22 (48), 7632-7641.
Datasheet 0802-W
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