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Detection Sets
Death Domain Receptor Detection Set
Background
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis can be induced by certain cytokines such as TNF-a and Fas ligand. Apoptosis-inducing signals are often mediated by receptors containing so-called “death domains” in their cytoplasmic domains. These proteins include DR3 (also known as TNFRSF25), DR4 (also known as TNFRSF10A), DR5 (also known as TNFRSF10B), and DR6 (also known as TNFRSF21). DR3 is highly expressed in the tissues enriched in lymphocytes including thymus and spleen and may play a role in regulating lymphocyte homeostasis. DR4 is also called TRAIL receptor-1 (TRAIL-R1) and is expressed in most of human tissues. DR5, also known as TRAIL-R2, is widely expressed in normal tissues and in many types of tumor cells. Like DR5, DR6 is also widely expressed, and has recently been suggested to play a role in the pathogenesis of Alzheimer’s disease.
Two decoy receptors for TRAIL have been identified and designated DcR1 (also known as TNFRSF10C) and DcR2 (TNSRSF10D). DcR1 is a glycophospholipid-anchored cell surface protein with an extracellular TRAIL-binding domain but lacking an intracellular signaling domain. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis. DcR2 also has an extracellular TRAIL-binding domain but lacks the intracellular death domain and does not induce apoptosis. Similar to DcR1, overexpression of DcR2 attenuated TRAIL-induced apoptosis. Another decoy receptor, DcR3 (also known as TNFRSF6B) exists as a soluble rather than a membrane associated molecule. DcR3 binds to FasL and LIGHT and inhibits FasL and LIGHT induced apoptosis.
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Description
For images please see PDF data sheet
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Kit Contents
DR3 Antibody (CT), Catalog No. 1120 (50μg)
DR4 Antibody (CT), Catalog No. 1139 (50μg)
DR5 Antibody (CT), Catalog No. 2019 (50μg)
DR6 Antibody (NT), Catalog No. 2157 (50μg)
DcR1 Antibody (ED), Catalog No. 2179 (50μg)
DcR2 Antibody (ID), Catalog No. 2021 (50μg)
DcR3 Antibody (NT), Catalog No. 2140 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of DR3, DR4, DR5, DR6, DcR1, DcR2, and DcR3 by immunoblot at 1 – 2 μg/ml. DR4, DR5, DcR1, DcR2, and DcR3 can detect their respective proteins via immunohistochemistry at 1 – 10 μg/ml.
Tested Application
WB, IHC
Blocking Peptide
DR3 Peptide (CT), Catalog No. 1120P
DR4 Peptide (CT), Catalog No. 1139P
DR5 Peptide (CT), Catalog No. 2019P
DR6 Peptide (NT), Catalog No. 2157P
DcR1 Peptide (ED), Catalog No. 2179P
DcR2 Peptide (ID), Catalog No.2021P
DcR3 Peptide (NT), Catalog No. 2140P
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -20˚C.
Positive Control
- DR3 Antibody (CT): Jurkat Cell Lysate, Catalog No. 1205
DR4 Antibody (CT): HeLa Cell Lysate, Catalog No. 1201
DR5 Antibody (CT): HeLa Cell Lysate, Catalog No. 1201
DR6 Antibody (NT): K562 Cell Lysate, Catalog No. 1205
DcR1 Antibody (ED): HeLa Cell Lysate, Catalog No. 1201
DcR2 Antibody (ID): HeLa Cell Lysate, Catalog No. 1201
DcR3 Antibody (NT): Human Heart Tissue Lysate, Catalog No. 1301
Species Reactivity
see datasheet
Short Description
DR3 (CT), DR4 (CT), DR5 (CT), DR6 (NT), DcR1 (ED), DcR2 (ID), DcR3 (NT)
This product belongs to the following categories:
Related Products
References- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Siegel RM and Lenardo MJ. Apoptosis signaling pathways. Curr. Protoc. Immunol. 2002; Chapter 11.
- Chinnaiyan AM, O'Rourke K, Yu GL, et al. Signal transduction by DR, a death domain-containing receptor related to TNFR-1 and CD95. Science, 1996; 274:990-2.
- Pan G, O'Rourke K, Chinnaiyan AM, et al. The receptor for the cytotoxic ligand TRAIL. Science 1997; 276:111-113.
Datasheet 0801-W
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