|
Primary Antibodies
DcR3 Antibody
Background
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. Several novel members in the TNFR family including DR3, DR4, DR5, and DR6 were recently discovered and function as cell death receptors. Two decoy receptors, DcR1 and DcR2, were recently identified to compete with DR4 and DR5 for their ligand TRAIL binding. A novel decoy receptor was more recently discovered and designated DcR3 and TR6, respectively, (1,2). Unlike DcR1 and DcR2, DcR3 is a soluble rather than a membrane associated molecule. DcR3 binds to FasL and LIGHT and inhibits FasL and LIGHT induced apoptosis (1,2). DcR3 transcript is expressed in a number of lung and colon carcinomas and in some normal tissues.
Additional Names
DcR3, DCR3, TR6
|
Description
Left: Western blot analysis of DcR3 in human heart (H), brain (B), and kidney (K) tissue lysates with DcR3 antibody at 1:500 dilution.
Below: Immunohistochemistry of DcR3 in human heart tissue with DcR3 antibody at 1 μg/ml.
|
|
Other Product Images
|
Source
DcR3 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human DcR3 precursor.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
DcR3 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Human DcR3 (N-Terminus) Peptide (Cat. No. 2140P)
Application
DcR3 antibody can be used for detection of DcR3 expression by Western blot at 1:500 to 1:1000 dilution. An approximately 33 kDa band can be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2140P - DcR3 Peptide
Storage
DcR3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
4106877
Protein Accession Number
AF104419
Short Description
Decoy Receptor for FASL and LIGHT
This product belongs to the following categories:
AntibodiesPolyclonal AntibodiesApoptosis Antibodies
Related Products
References- Pitti RM, Marsters SA, Lawrence DA, Roy M, Kischkel FC, Dowd P, Huang A, Donahue CJ, Sherwood SW, Baldwin DT, Godowski PJ, Wood WI, Gurney AL, Hillan KJ, Cohen RL, Goddard AD, Botstein D, Ashkenazi A. Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer. Nature 1998;396:699-703
- Yu KY, Kwon B, Ni J, Zhai Y, Ebner R, Kwon BS. A newly identified member of tumor necrosis factor receptor superfamily (TR6) suppresses LIGHT-mediated apoptosis. J Biol Chem 1999;274:13733-6 (RD1299)
Datasheet 08-02W
|
|
