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Primary Antibodies
DcR2 Antibody
Background
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL, and DcR1/TRID is a decoy receptor (1-3). Another member of the TRAIL receptor family was more recently identified and designated DcR2, TRAIL-R4, or TRUNDD (4-6). DcR2 has an extracellular TRAIL-binding domain but lacks intracellular death domain and does not induce apoptosis. Like DR4 and DR5, DcR2 transcript is widely expressed in normal human tissues. Overexpression of DcR2 attenuated TRAIL-induced apoptosis.
Additional Names
DcR2 (ID), TRAIL-R4
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Description
Left: Western blot analysis of DcR2 in HeLa whole cell lysate with DcR2 antibody at 1:1000 dilution.
Below: Immunocytochemistry staining of HeLa cells using DcR2 antibody at 10μg/ml.
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Other Product Images
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Source
DcR2 antibody was raised against a peptide corresponding to amino acids 249 to 263 of human DcR2 precursor.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DcR2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DcR2 (Intracellular Domain) Peptide (Cat. No. 2021P)
Application
DcR2 antibody can be used for detection of DcR2 expression by Western blot at 1:1000 to 1:2000 dilution.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2021P - DcR2 Peptide
Storage
DcR2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
18203495
Protein Accession Number
Q9UBN6
Short Description
(ID) Decoy Receptor 2 for TRAIL
This product belongs to the following categories:
Related Products
References- Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113
- Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8
- Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21
- Marsters SA, Sheridan JP, Pitti RM, Huang A, Skubatch M, Baldwin D, Yuan J, Gurney A, Goddard AD, Godowski P, Ashkenazi A. A novel receptor for Apo2L/TRAIL contains a truncated death domain. Curr Biol 1997;7:1003-6
Datasheet 08-02W
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