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Primary Antibodies
DcR1 Antibody
Background
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3). Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT (2-7) and DcR2/TRAIL-R4/TRUNDD (8-10). DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts are expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.
Additional Names
DcR1, DcR1 (ED2), TRAIL-R3, TRID, LIT
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Description
Left: Western blot analysis of DcR1 in HeLa cell (A), mouse (B) and rat (C) liver tissue lysates with DcR1 antibody at 1 μg/ml.
Below: Immunofluorescence of DcR1 in rat liver tissue with DcR1 antibody at 10 μg/ml.
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Other Product Images
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Source
DcR1 antibody was raised against a peptide corresponding to amino acids in the extracellular domain of human DcR1 precursor.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DcR1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DcR1 Peptide (Cat. No. 2299P)
Application
DcR1 antibody can be used for detection of DcR1 by Western blot at 0.5 to 1 μg/ml. An approximate 65 kDa band can be detected.
Tested Application
E, WB, IF
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2299P - DcR1 Peptide
Storage
DcR1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
- Cat. No. 1404 - Mouse Liver Tissue Lysate
- Cat. No. 1464 - Rat Liver Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
2338422
Protein Accession Number
AAB67104
Short Description
(ED2) Decoy Receptor 1 for TRAIL
This product belongs to the following categories:
Related Products
References- Pan G; O'Rourke K; Chinnaiyan et al.. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113
- Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8
- Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21
- Degli-Esposti MA, Smolak PJ, Walczak H, et al, Smith CA. Cloning and characterization of TRAIL-R3, a novel member of the emerging TRAIL receptor family. J Exp Med 1997;186(7):1165-70
Datasheet 08-02W
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