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Primary Antibodies
DcR1 Antibody
Background
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL. Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.
Additional Names
DcR1 (ED), TRAIL-R3
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Description
Left: Western blot analysis of DcR1 in HeLa whole cell lysate with DcR1 antibody (ED) at 1:500 dilution.
Below: Immunocytochemistry of DcR1 in HeLa cells with DcR1 antibody at 10 μg/ml.
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Other Product Images
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Source
DcR1 antibody was raised against a peptide corresponding to amino acids in an extracellular domain (ED) of human DcR1 precursor.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DcR1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DcR1 (Extracellular Domain) Peptide (Cat. No. 2179P)
Application
DcR1 antibody can be used for detection of DcR1 by Western blot at 1:500 to 1:1000 dilution. An approximate 65 kDa band can be detected (6). It is human, mouse, and rat reactive.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2179P - DcR1 Peptide
Storage
DcR1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
2338421
Protein Accession Number
AF012536
Short Description
(ED) Decoy Receptor 1 for TRAIL
This product belongs to the following categories:
Related Products
References- Pan G; O'Rourke K; Chinnaiyan et al.. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113
- Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8
- Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21
- Degli-Esposti MA, Smolak PJ, Walczak H, et al, Smith CA. Cloning and characterization of TRAIL-R3, a novel member of the emerging TRAIL receptor family. J Exp Med 1997;186(7):1165-70
Datasheet 08-02W
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