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Catalog Number:

XAV-8295


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

DbpA/CSDA Antibody

Background

Human DNA-binding protein (dbpA) is a member of a Y-box binding protein family containing a cold shock domain. The increased expression of Y box binding proteins in somatic cells is associated with cell proliferation and transformation.

DbpA/CSDA Antibody

Description

Left: Western blot analysis of DbpA/CSDA expression in Raji (A) and 293T (B) cell lysates using anti-DbpA/CSDA.

Purification

Rabbit IgG is purified by Protein A/G affinity chromatography method.

Clonality / Clone

This is a polyclonal antibody.

Host

DbpA/CSDA antibody was raised in rabbit.

Please use anti-rabbit IgG secondary antibodies.

Immunogen

DbpA/CSDA antibody was produced in rabbits immunized with a synthetic peptide corresponding to a region of human CSDA.

Application

DbpA/CSDA antibody can be used for Western blot at a suggested dilution of 1 μg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. The protein size is 372 amino acids.

Tested Application

WB

Buffer

Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.

Storage

DbpA/CSDA antibody is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ºC. Avoid repeat freeze-thaw cycle.

Positive Control

  1. Cat. No. 1207 - Raji Cell Lysate
  2. Cat. No. 1210 - 293 Cell Lysate

Species Reactivity

H

Protein Accession Number

NP_003642

Molecular Weight

32/37/40 kDa

Short Description

Anti-DbpA/CSDA

This product belongs to the following categories:

Related Products

References

  1. Hayashi, J. et al, (2002) Int. J. Oncol. 21 (4), 847-850


Datasheet 0802-W

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