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Primary Antibodies
DRAK2 Antibody
Background
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues (1).
Additional Names
DRAK2 (CT), DRAK2
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Description
Left: Western blot analysis of DRAK2 in Jurkat (1,3) and Raji (2,4) cell lysate in the absence (1,2) or presence (3,4) of blocking peptide with DRAK2 antibody at 1:500 dilution.
Below: Immunocytochemistry of DRAK2 in Jurkat cells with DRAK2 antibody at 10 μg/ml.
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Other Product Images
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Source
DRAK2 antibody was raised against a peptide corresponding to amino acids 351 to 365 of human DRAK2.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
DRAK2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DRAK2 (C-Terminus) Peptide (Cat. No. 2149P)
Application
DRAK2 antibody can be used for detection of DRAK2 by Western blot at 1:500 to 1:1000 dilution. An approximately 45 kDa band can be detected. It has no cross responses to DAP or ZIP kinases. The approximately 70 kDa band is probably non-related to DRAK2 although it is peptide blockable.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2149P - DRAK2 Peptide
Storage
DRAK2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Protein GI Number
3834355
Protein Accession Number
AB011421
Short Description
(CT) DAPK Related Apoptosis-inducing Kinase
This product belongs to the following categories:
Related Products
References- Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71 (RD1299)
Datasheet 08-02W
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