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Primary Antibodies
DRAK1 Antibody
Background
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 for DAP kinase-related apoptosis-inducing protein kinases (1). DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK1 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK1 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues (1).
Additional Names
DRAK1 (NT), DRAK1
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Description
Left: Western blot analysis of DRAK1 in (A) MOLT4 and (B) A431 whole cell lysates with DRAK1 antibody at 1 μg/ml.
Below: Immunocytochemistry staining of MOLT4 cells using DRAK1 antibody at 2 μg/ml.
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Other Product Images
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Source
DRAK1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human DRAK1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DRAK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DRAK1 (N-Terminus) Peptide (Cat. No. 2147P)
Application
DRAK1 antibody can be used for detection of DRAK1 by Western blot at 0.5 – 1 μg/ml. A431 or MOLT4 whole cell lysate can be used as positive control and an approximately 50 kDa band can be detected. DRAK1 antibody is human specific, and has no cross responses to DRAK2, DAP or ZIP kinases.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2147P - DRAK1 Peptide
Storage
DRAK1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1202 - A431 Cell Lysate
- Cat. No. 1206 - MOLT4 Cell Lysate
Species Reactivity
H
Protein GI Number
14423930
Protein Accession Number
Q9UEE5
Short Description
(NT) DAPK Related Apoptosis-inducing Kinase
This product belongs to the following categories:
Related Products
References- Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71
Datasheet 09-01W
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