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Primary Antibodies
DR6 (MAb Luke-1) Antibody
Background
*Please allow 3-5 days for delivery time.*
Apoptosis is induced by certain cytokines including TNF and Fas ligand (CD95L) of the TNF family through their death domain containing receptors, TNF-R1 and Fas (CD95). Several novel death receptors including DR3, TRAIL-R1, and TRAIL-R2 were identified. A new death domain containing receptor in the TNF-R family was cloned and termed DR6 for death receptor-6. Like TNF-R1, DR6 interacts with death domain containing adapter molecule TRADD. Overexpression of DR6 induces apoptosis and activates NF-?B and JNK [1]. DR6 is widely expressed in human tissues and cell lines. The ligand for DR6 has not been identified yet. T cells from DR6-deficient mice showed preference toward Th2 differentiation in vitro, suggesting that DR6, working through JNK functions to attenuate the Th2 response [2]. Other studies of DR6-targeted mutant mice demonstrated that DR6 functions as a regulatory receptor for mediating CD4(+) T cell activation and maintaining proper immune responses [3].
Additional Names
DEATH RECEPTOR-6, TNFRSF 21
Source
DR6 (monoclonal Luke-1) antibody was raised against recombinant human DR6;Fc (aa. 42-335).
Purification
DR6 (monoclonal Luke-1) antibody was purified to ≥95% in SDS-PAGE and is supplied at a concentration of 1mg/1ml.
Clonality / Clone
This is a monoclonal antibody.
Isotype
Rat IgG2a
Host
DR6 antibody was raised in rat.
Please use anti-rat IgG secondary antibodies
Immunogen
The immunogen is recombinant human DR6;Fc, amino acids 42-335.
Application
DR6 antibody can be used to recognize human DR6 by Flow Cytometry. Method: Cells (5x105) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1µg/ml of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mDR6 (thick line). Cells were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg/ml of Luke-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
Tested Application
FC
Buffer
DR6 antibody is supplied as a liquid in PBS containing 0.02% sodium azide.
Storage
DR6 antibody can be stored at 4˚C, stable for one year.
Species Reactivity
H
Short Description
monoclonal to DR6 (Luke-1)
This product belongs to the following categories:
References- Identification and functional characterization of DR6, a novel death domain-containing TNF receptor : G. Pan, et al.; FEBS Lett 431, 351 (1998).
- Impaired c-Jun amino terminal kinase activity and T cell differentiation in death receptor 6-deficient mice : H. Zhao, et al.; J. Exp. Med. 194, 1441 (2001).
- Enhanced CD4+ T cell proliferation and Th2 cytokine production in DR6-deficient mice:J. Liu, et al.; Immunity 15, 23 (2001).
Datasheet 0802-W
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