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Primary Antibodies
DR5 Antibody
Background
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-7). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-κB.
Additional Names
DR5, TRAIL-R2
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Description
Left: Western blot analysis of DR5 in HeLa (H) and K562 (K) cell lysates with DR5 antibody at 2 μg/ml.
Below: Immunocytochemistry of DR5 in HeLa cells with DR5 antibody at 5 μg/ml.
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Other Product Images
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Source
DR5 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DR5 precursor .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DR5 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DR5 (C-Terminus) Peptide (Cat. No. 2019P)
Application
DR5 antibody can be used for detection of DR5 expression by Western blot at 1:250 to 1:500 dilution. antibody has no cross reaction to DR4.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2019P - DR5 Peptide
Storage
DR5 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H, M
Protein GI Number
2338419
Protein Accession Number
AF012535
Short Description
Death Receptor for TRAIL
This product belongs to the following categories:
Related Products
References- Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8
- Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21
- Walczak H, Degli-Esposti MA, Johnson RS, Smolak PJ, Waugh JY, Boiani N, Timour MS, Gerhart MJ, Schooley KA, Smith CA, Goodwin RG, Rauch CT. TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL. EMBO J 1997;16:5386-97
- MacFarlane M, Ahmad M, Srinivasula SM, Fernandes-Alnemri T, Cohen GM, Alnemri ES. Identification and molecular cloning of two novel receptors for the cytotoxic ligand TRAIL. J Biol Chem 1997;272:25417-20
Datasheet 08-02W
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