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Primary Antibodies
DR4 Antibody
Background
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4). The ligand for this novel death receptor has been identified and termed TRAIL2,3, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-κB activation in many tissues and cells.
Additional Names
DR4 (CT), TRAIL-R1
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Description
Left: Western blot analysis of DR4 in HeLa total cell lysate with DR4 antibody at 1:500 dilution.
Below: Immunocytochemistry of DR4 in Jurkat cells with DR4 antibody at 10 μg/ml.
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Other Product Images
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Source
DR4 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DR4 protein.
Purification
Antibody is DEAE purified
Clonality / Clone
This is a polyclonal antibody.
Host
DR4 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DR4 (C-Terminus) Peptide (Cat. No. 1139P)
Application
DR4 antibody can be used for detection of DR4 expression by Western blot at 1:500 to 1:1000 dilution. A 57 kDa band should be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 1139P - DR4 Peptide
Storage
DR4 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H
Protein GI Number
1945072
Protein Accession Number
AAC51226
Short Description
(CT) Death Receptor for TRAIL
This product belongs to the following categories:
Related Products
References- Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113
- Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch C, Smith CA, et al. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 1995;3:673-682
- Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A; Ashkenazi A. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem. 1996;271:12687-90
- Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K, Kataoka T, Holler N, Tschopp J. TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF-κB. Immunity 1997;7:831-836 (RD1299)
Datasheet 08-02W
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