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Primary Antibodies
DR3 Antibody
Background
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD1-5. The ligand for this novel death receptor has been defined as TWEAK, also termed Apo3L. DR3 is highly expressed in the tissues enriched in lymphocytes including PBL, thymus and spleen. Like TNFR1, DR3 induces apoptosis and NF-κB activation.
Additional Names
DR3 (ED), Wsl-1 Apo-3
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Description
Left: Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:1000 dilution.
Below: Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 μg/ml.
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Other Product Images
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Source
DR3 antibody was raised against a peptide corresponding to amino acids in extracellular domain (ED) of human DR3 precusor.
Purification
Antibody is DEAE purified
Clonality / Clone
This is a polyclonal antibody.
Host
DR3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Bacteria DR3 (Extracellular Domain) Peptide (Cat. No. 1158P)
Application
DR3 antibody can be used for detection of DR3 expression by Western blot at 1:500 to 1:1000 dilution. 59 kDa band should be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 1158P - DR3 Peptide
Storage
DR3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H, M
Protein GI Number
37181738
Protein Accession Number
AAQ88676
Short Description
(ED) Cell Death Receptor
This product belongs to the following categories:
Related Products
References- Chinnaiyan AM; O'Rourke K; Yu GL; Lyons RH; Garg M; Duan DR; Xing L; Gentz R; Ni J; Dixit VM. Science, 1996;274:990-2.
- Kitson J; Raven T; Jiang YP; Goeddel DV; Giles KM; Pun KT; Grinham CJ; Brown R; Farrow SN. Nature, 1996;384:372-5.
- Marsters SA; Sheridan JP; Donahue CJ; Pitti RM; Gray CL; Goddard AD; Bauer KD; Ashkenazi A. Curr Biol, 1996;6:1669-76.
- Bodmer JL; Burns K; Schneider P; Hofmann K; Steiner V; Thome M; Bornand T; Hahne M; Schroter M; Becker K; et al. Immunity, 1997;6:79-88.
Datasheet 08-02W
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