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Primary Antibodies
DOK1 Antibody
Background
Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.
Additional Names
DOK1 (CT), DOK1
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Description
Left: Western blot analysis of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 μg/ml.
Below: Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 μg/ml.
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Other Product Images
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Source
DOK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DOK1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DOK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DOK Peptide (Cat. No. 1107P)
Application
DOK1 antibody can be used for detection of DOK1 expression by Western blot at 0.5 - 1 μg/ml. A 62 kDa band should be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 1107P - DOK1 Peptide
Storage
DOK1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
- Cat. No. 1208 - THP-1 Cell Lysate
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H
Protein GI Number
1848277
Protein Accession Number
AAC51127
Short Description
(CT) Tyrosine kinase substrate DOK
This product belongs to the following categories:
Related Products
References- Carpino N, Wisniewski D, Strife A, Marshak D, Kobayashi R, Stillman B, Clarkson B p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Cell 1997;88:197-204.
- Yamanashi Y, Baltimore D Identification of the Abl- and rasGAP-associated 62 KDa protein as a docking protein, Dok. Cell 1997;88:205-211.
- Holland SJ, Gale NW, Gish GD, Roth RA, Songyang Z, Cantley LC, Henkemeyer M, Yancopoulos GD, Pawson T. Juxtamembrane tyrosine residues couple the Eph family receptor EphB2/Nuk to specific SH2 domain proteins in neuronal cells. EMBO J 1997;16:3877-3888.
- Di Cristofano A, Carpino N, Dunant N, Friedland G, Kobayashi R, Strife A, Wisniewski D, Clarkson B, Pandolfi PP, Resh MD. Molecular cloning and characterization of p56(dok-2) defines a new family of RasGAP-binding proteins. J Biol Chem 1998;273:4827-4830.
Datasheet 08-02W
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