|
Primary Antibodies
DNAL1 Antibody
Background
DNAL1 was identified as a potential candidate gene for primary ciliary dyskinesia (PCD), a genetically heterologous disorder characterized by chronic infections of the upper and lower airways that often leads to permanent lung damage, randomization of left/right body symmetry, and reduced fertility. DNAL1 is reported to be expressed solely in tissues carrying motile cilia for flagella and interacts with DNAH5, a protein that when mutated has been shown to result in PCD. It has been suggested that DNAL1 serves a regulatory function for DNAH5 activity in outer dynein arms of sperm flagella, respiratory cilia, and ependymal cilia. DNAL1 has also been recently identified as an HIV dependency factor (HDF), suggesting that DNAL1 may be an important drug target in HIV treatment. At least two isoforms of DNAL1 are known to exist.
Additional Names
DNAL1, Dynein axonemal light chain 1
|
Description
Left: Western blot analysis of DNAL1 in 3T3 cell lysate with DNAL1 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunocytochemistry of DNAL1 in 3T3 cells with DNAL1 antibody at 2.5 μg/ml.
|
|
Other Product Images
|
Source
DNAL1 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human DNAL1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DNAL1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
DNAL1 antibody can be used for detection of DNAL1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4733P - DNAL1 Peptide
Storage
DNAL1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
164607156
Protein Accession Number
NP_113615
Short Description
Dynein axonemal light chain 1
This product belongs to the following categories:
Related Products
References- Horvath J, Fliegauf M, Olbrich H, et al. Identification and analysis of axonemal dynein light chain 1 in primary ciliary dyskinesia patients. Am. J. Respir. Cell Mol. Biol. 2005; 33:41-7.
- Olbrich H, Haeffner K, Kisbert A, et al. Mutations in DNAH5 cause primary ciliary dyskinesia and randomization of left/right asymmetry. Nat. Genet. 2002; 30:143-4.
- Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 2008; 319:921-6.
Datasheet 08-04W
|
|
