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Primary Antibodies
DFF40 Antibody
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively, (3-5). DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6) and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
Additional Names
DFF40, DFF40 (I18), CAD (I18)
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Description
Left: Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.
Below: Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 μg/ml.
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Other Product Images
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Source
DFF40 antibody was raised against a peptide corresponding to amino acids near the center of murine CAD. The sequence differs from human DFF40 by two amino acids (3,4).
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DFF40 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DFF40 Peptide (Cat. No. 2107P)
Application
DFF40 antibody can be used for detection of DFF40 by Western blot at 1:500 to 1:1000 dilution. A 40 kDa band can be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2107P - DFF40 Peptide
Storage
DFF40 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
- Cat. No. 1205 Jurkat Whole Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
160948620
Protein Accession Number
NP_031885
Short Description
Caspase Activated DNase
This product belongs to the following categories:
Related Products
References- Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
- Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
- Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40
- Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6
Datasheet 08-02W
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