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Primary Antibodies
DEDAF Antibody
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor . DEDAF is identical to the transcriptional repressor RYBP (2). DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
Additional Names
DEDAF
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Description
Left: Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 μg /ml.
Below: Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 10 μg/ml.
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Other Product Images
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Source
DEDAF antibody was raised against a synthetic peptide corresponding to amino acids 215 to 228 of human DEDAF. The sequence is identical to that of mouse origin.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DEDAF antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DEDAF Peptide (Cat. No. 2227P)
Application
DEDAF can be used for detection of DEDAF by Western blot at 0.5 to 1 μg/ml. A band at approximately 32 kDa can be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
DEDAF antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1203 - A549 Cell Lysate
- Cat. No. 1211 - HepG2 Whole Cell Lysate
- Cat. No. 1404 - Mouse Liver Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
5802963
Protein Accession Number
AF179286
This product belongs to the following categories:
Related Products
References- Zheng L, Schickling O, Peter ME, Lenardo MJ. The death effector domain-associated factor (DEDAF) plays distinct regulatory roles in the nucleus and cytoplasm. J Biol Chem. 2001;276(34):31945-52.
- Garcia E, Marcos-Gutierrez C, del Mar Lorente M, Moreno JC, Vidal M. RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. EMBO J. 1999;18(12):3404-18. (RD0201)
Datasheet 08-02W
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