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Primary Antibodies
DDR2 Monoclonal Antibody
Background
DDR2 (discoidin domain receptor family, member 2) is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/ threonine (STK) kinase catalytic domains. Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein.
Additional Names
TKT, MIG20a, NTRKR3, TYRO10
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Description
Western blot analysis using DDR2 monoclonal antibody against truncated DDR2 - Trx recombinant protein.
Below: Innunofluorescense staining of methanol - fixed Gc - 7901 cells showing cytoplasm localization.
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Clonality / Clone
This is a monoclonal antibody. Clone 3B11E4.
Isotype
IgG2a
Host
DDR2 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Ni-NTA purified truncated recombinant DDR2-Trx expressed in E. Coli strain BL21 (DE3).
Application
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Tested Application
E, WB, IHC
Storage
DDR2 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H
Formulation
Ascitic fluid containing 0.03% sodium azide.
Purified antibody available at no extra cost.
Protein GI Number
4921
Short Description
discoidin domain receptor family, member 2
This product belongs to the following categories:
References- Leitinger B. Kwan AP. Matrix Biol. 2006, Aug, 25(6):355-64. Epub 2006 May 26.
- Shyu KG. Chao YM. Wang BW. et al. Hypertension. 2005, Sep, 46(3):614-21. Epub 2005 Aug 8.
- Neale JC. Kenny TP. Gershwin ME. Clin Dev Immunol. 2004, Jun, 11(2):157-63.
Datasheet 0903-W
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