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Primary Antibodies
DDC Antibody
Background
DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.
Additional Names
DDC, AADC
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Description
Left: Western blot analysis of DDC in HepG2 Cell Lysate using DDC antibody at 5 μg/ml (12% gel).
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Purification
Antibody is purified by protein A chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
DDC antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDC.
Application
DDC antibody can be used for detection of DDC by ELISA at 1:312500. DDC antibody can be used for detection of DDC by western blot at 5.0 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB, IHC
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store DDC antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H, M, D
Protein Accession Number
NP_000781
Molecular Weight
53kDa, 54kDa
Short Description
DDC (dopa decarboxylase (aromatic L-amino acid decarboxylase))
This product belongs to the following categories:
References- Ma,J.Z., (2005) Hum. Mol. Genet. 14 (12), 1691-1698.
Datasheet 10-01W
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