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Primary Antibodies
DC-SIGN Monoclonal Antibody
Background
Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes (1), where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently (4-8). DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR (8). The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.
Additional Names
DC-SIGN (5D7), Dendritic cell-specific ICAM-3-grabbing nonintegrin 1
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Description
Left: Western blot detection of DC-SIGN fusion protein in human placenta at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of DC-SIGN in lymph node tissue with DC-SIGN antibody at 5 μg/ml.
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Other Product Images
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Source
Mouse monoclonal DC-SIGN antibody was raised against a recombinant His-tagged protein fragment corresponding to the extracellular region of human DC-SIGN (1).
Purification
Immunoaffinity chromotography purified IgG
Clonality / Clone
This is a monoclonal antibody. (Clone 5D7)
Host
DC-SIGN monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
A peptide corresponding to amino acids near the carboxy terminus of human DC-SIGN.
Application
DC-SIGN antibody can be used for detection of DC-SIGN in immunoblots at 1 – 2 mg/ml and in immunohistochemistry at 5 – 10 mg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
DC-SIGN monoclonal antibody can be stored at 4˚C, stable for one year.
Positive Control
- Cat. No. 1384 - Human Uterus Tissue Lysate
- Cat. No. 1369 - Human Lymph Node Tissue Lysate
Species Reactivity
H
Short Description
HIV binding receptor
This product belongs to the following categories:
Related Products
References- Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 2000; 100:587-97.
- Pohlmann S, Baribaud F, Lee B, et al. DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus. J. Virol. 2001;75:4664-72.
- Geijtenbeek TB, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell 2000; 100:575-85.
- Soilleux EJ, Barten R, Trowsdale J. DC-SIGN; a related gene, DC-SIGNR; and CD23 form a cluster on 19p13. J. Immunol. 2000; 165:2937-42.
Datasheet 0803-W
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