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Primary Antibodies
DC-SIGN Antibody
Background
Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes (1), where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently (4-8). DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR (8). The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.
Additional Names
DC-SIGN (ED), DC-SIGN
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Description
Left: Western blot analysis of DC-SIGN expression in human placenta tissue lysate in the absence (lane 1) and presence (lane 2) of blocking peptide with DC-SIGN antibody at 2 μg /ml.
Below: Immunohistochemistry of DC-SIGN in human small intestine tissue with DC-SIGN antibody at 10 μg/ml.
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Other Product Images
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Source
DC-SIGN antibody was raised against a synthetic peptide corresponding to amino acids near the center of human DC-SIGN .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DC-SIGN antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DC-SIGN (Extracellular Domain) Peptide (Cat. No. 2349P)
Application
DC-SIGN antibody can be used for detection of DC-SIGN by Western blot at 1 – 2 μg/ml. A band at approximately 44 kDa can be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2349P - DC-SIGN Peptide
Storage
DC-SIGN antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1309 - Human Placenta Lysate
- Cat. No. 1308 - Human Small Intestine Tissue Lysate
Species Reactivity
H
Protein GI Number
46396012
Protein Accession Number
Q9NNX6
Short Description
(ED) A novel HIV biding protein
This product belongs to the following categories:
Related Products
References- Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell. 2000;100:587-97.
- Pohlmann S, Baribaud F, Lee B, et al. RW. DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus. J Virol. 2001;75(10):4664-72.
- Geijtenbeek TB, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell. 2000;100(5):575-85.
- Soilleux EJ, Barten R, Trowsdale J. DC-SIGN; a related gene, DC-SIGNR; and CD23 form a cluster on 19p13. J Immunol. 2000;165(6):2937-42.
Datasheet 08-02W
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