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Primary Antibodies
DARC Antibody
Background
DARC, also known as the Duffy antigen/chemokine receptor, is a seven-transmembrane protein homologous to the classical chemokine G-protein coupled receptors (GPCRs) with the exception of the motif required for G protein coupling. DARC can bind with high affinity several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. Recently, DARC was found to interact with KAI1, a four transmembrane protein recently identified as a tumor metastasis suppressor protein. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least three isoforms of DARC are known to exist.
Additional Names
DARC (NT), Duffy antigen, Fy glycoprotein, GpFy, Cell adhesion molecule 3, CADM3
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Description
Left: Western blot analysis of DARC in PC-3 cell lysate with DARC antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunohistochemistry of DARC in human brain tissue with DARC antibody at 5 μg/ml.
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Other Product Images
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Source
DARC antibody was raised against a 18 amino acid peptide from near the amino terminus of human DARC.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DARC antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
DARC antibody can be used for detection of DARC by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4071P - DARC Peptide
Storage
DARC antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1216 - PC-3 Cell Lysate
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H
Protein GI Number
67476970
Protein Accession Number
Q16570
Short Description
(NT) Duffy antigen/chemokine receptor
This product belongs to the following categories:
Related Products
References- Chaudhuri A, Polyakova J, Zbrzezna V, et al. Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite. Proc. Natl. Acad. Sci. USA 1993; 90:10793-7.
- Gardner L, Patterson AM, Ashton BA, et al. The human Duffy antigen binds selected inflammatory but not homeostatic chemokines. Biochem. Biophys. Res. Commun. 2004; 321:306-12.
- Gil ML, Vita N, Lebel-Binay S, et al. A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and functional studies. J. Immunol. 1992; 2826-33.
Datasheet 08-02W
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