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Primary Antibodies
DAPK2 Antibody
Background
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNg induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1 (1, 2). Ectopicly expressed DAPK2 induced apoptosis in various types of cells (1,2). DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues (1).
Additional Names
DAPK2
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Description
Left: Western blot analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody at 1 μg/ml.
Below: Immunohistochemistry of DAPK2 in mouse spleen cells with DAPK2 antibody at 2 μg/ml.
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Other Product Images
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Source
DAPK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2. The sequence of this antigenic peptide is identical to the corresponding amino acids of mouse origin (1,2).
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
DAPK2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human DAP kinase 2 Peptide (Cat. No. 2323P)
Application
DAPK2 antibody can be used for detection of DAPK2 by Western blot at 0.5 to 1 μg/ml. An approximately 42 kDa band can be detected. DAPK2 has no cross responses to DAPK1.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2323P - DAPK2 Peptide
Storage
DAPK2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1202 - A431 Cell Lysate
- Cat. No. 1406 - Mouse Spleen Tissue Lysate
- Cat. No. 1465 - Rat Kidney Lysate
Species Reactivity
H, M, R
Protein GI Number
6521210
Protein Accession Number
BAA88063
Short Description
Death-Associated Protein kinase 2
This product belongs to the following categories:
Related Products
References- Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ, Jenkins NA, Akira S. Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity. Oncogene 1999;18(23):3471-80
- Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A. Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis. Mol Cell Biol 2000;20(3):1044-54 (WD0101)
Datasheet 08-02W
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