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Primary Antibodies
DAD1 Antibody
Background
Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death (1), a process important for normal organism development and tissue homeostasis (2). DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity (3,4). Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death (5,6). Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas (7), indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
Additional Names
DAD1
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Description
Left: Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/ml.
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Other Product Images
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Source
DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
DAD1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Murine DAD1 Peptide (Cat. No. 3313P)
Application
DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/ml. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3313P - DAD1 Peptide
Storage
DAD1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1211 - HepG2 Cell Lysate
Species Reactivity
H, M
Protein GI Number
14602573
Protein Accession Number
AAH09798
Short Description
An inhibitor of apoptosis
This product belongs to the following categories:
Related Products
References- Nakashima T, Sekiguchi T, Kuraoka A, et al. Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. Mol. Cell Biol. 1993; 13:6367-74.
- Stellar H. Mechanisms and genes of cellular suicide. Science 1995; 267:1445-9.
- Gilmore R and Kelleher DJ. DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase. Proc. Natl. Acad. Sci. USA 1997; 94:4994-9.
- Sanjay A, Fu J, and Kreibich G. DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex. J. Biol. Chem. 1998; 273:26094-9.
Datasheet 08-02W
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