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Primary Antibodies
Cyclooxygenase-2 Antibody
Background
COX2 (Cyclooxygenase-2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate-early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged. Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recognizes rat COX2. Species Crossreactivity: Human and mouse, .
Prostaglandin-endoperoxide synthase (fatty acid cyclooxygenase; PGH synthase; Cox-2) is a key enzyme in prostaglandin biosynthesis. The first rate-limiting step in the conversion of arachidonic acid to prostaglandins is catalyzed by Cox-1 and Cox-2. The cyclooxygenase activity of Cox-2 is inhibited by nonsteroidal antiinflammatory drugs such as aspirin and endomethacin. It is a mitogen-inducible gene (unlike Cox-1, which is a house-keeping gene), and this means it probably is a primary mediator of the inflammatory response.
Additional Names
PTGS2, COX-2, COX2, EC 1.14.99.1, HCOX-2, PGG/HS, PGHS-2, PHS-2, cox-2, cox2, cyclooxygenase-2, pgh synthase 2, pghs-2, phs 2, prostaglandin g/h synthase 2, prostaglandin h2 synthase 2, prostaglandin-endoperoxide synthase 2, ptgs2
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Description
Left: Immunohistochemistry staining of Cyclooxygenase-2 in liver (formalin-fixed paraffin-embedded) using Cyclooxygenase-2 antibody.
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Purification
Immunoaffinity Chromatography
Clonality / Clone
This is a polyclonal antibody.
Host
Cyclooxygenase-2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Cyclooxygenase-2 antibody was raised against a synthetic peptide corresponding to the C-terminus of Cyclooxygenase-2. (Rat)
Application
Cyclooxygenase-2 antibody can be used for detection of Cyclooxygenase-2 by WB (minimum working concentration: 2 μg/ml maximum working concentration: 4 μg/ml), IP, ELISA, IHC-parafin (minimum working concentration: 5 μg/ml).
Tested Application
E, WB, IHC, IP
Buffer
Cyclooxygenase-2 antibody is supplied in 10 mM PBS, pH 7.6, 0.2% BSA, 15 mM sodium azide, before the addition of glycerol to 40%.
Storage
Cyclooxygenase-2 antibody can be stored at 4˚C or -20˚C, Avoid repeated freezing and thawing.
Species Reactivity
H, M
Formulation
Volume: 50 μl
Short Description
Cyclooxygenase-2
This product belongs to the following categories:
Datasheet 09-01W
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