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Primary Antibodies
Collagen VII Antibody
Background
Collagen VII Antibody. This product does not work on cryostat sections of Normal Human Skin.
Additional Names
COL7A1, collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive), HGNC:2214, EBD1, EBDCT, EBR1, LC collagen, alpha 1 type VII collagen, collagen VII, alpha-1 polypeptide, long chain collagen
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Description
Left: Western blot analysis of Collagen VII in rat testis lysate (35μg protein in RIPA buffer) using Collagen VII antibody (0.5μg/mL).
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Source
Collagen VII antibody was raised against a synthetic peptide of Collagen VII.
Purification
Collagen VII antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Collagen VII antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approx 250kDa band observed in rat testis lysates (calculated MW of 159kDa according to rat NP_001100328.1). Recommended concentration: 0.1-1.5μg/ml.
Tested Application
E, WB
Buffer
Collagen VII antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-647P - Collagen VII Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1476 - Rat Testis Tissue Lysate
Species Reactivity
H, M, R, D
Protein Accession Number
NP_000085.1
Short Description
Collagen, type VII
This product belongs to the following categories:
Related Products
References- Ortiz-Urda S, Garcia J, Green CL, Chen L, Lin Q, Veitch DP, Sakai LY, Lee H, Marinkovich MP, Khavari PA. Type VII collagen is required for Ras-driven human epidermal tumorigenesis. Science. 2005 Mar 18;307(5716):1773-6
Datasheet 09-01W
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