|
Primary Antibodies
Cofilin 2 Antibody
Background
Cofilin 2 Antibody. This antibody is expected to recognize both reported isoforms (NP_068733 and NP_619579)
Additional Names
CFL2, cofilin 2 (muscle) , HGNC:1875 , cofilin 2
|
Description
Left: Western blot analysis of cofilin 2 in human muscle lysate (35μg protein in RIPA buffer) using cofilin 2 antibody (0.003μg/mL).
Below: HEK293 overexpressing Cofilin 2 (muscle) and probed with cofilin 2 antibody (mock transfection in first lane).
|
|
Other Product Images
|
Source
Cofilin 2 antibody was raised against a synthetic peptide near the C-terminus of cofilin 2.
Purification
Cofilin 2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Cofilin 2 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approx 18kDa band observed in human duodenum, heart and muscle lysates (calculated MW of 18.7kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.01-0.03μg/ml
Tested Application
E, WB
Buffer
Cofilin 2 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-036P - Cofilin 2 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Species Reactivity
H, M, R, D, P
Protein Accession Number
NP_068733.1, NP_619579.1
Short Description
(CT) Cofilin 2 (muscle)
This product belongs to the following categories:
Related Products
References- Thirion C, Stucka R, Mendel B, Gruhler A, Jaksch M, Nowak KJ, Binz N, Laing NG, Lochmuller H. Characterization of human muscle type cofilin (CFL2) in normal and regenerating muscle. Eur J Biochem. 2001 Jun;268(12):3473-82.
Datasheet 08-02W
|
|
