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Primary Antibodies
Clusterin Monoclonal Antibody
Background
Clusterin (SGP-2, SP-40,40, pADHC-9, TRPM-2, CLJ, T64, GP III, APO J, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in several physiological processes. Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has a number of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress-induced insults caused by degraded and misfolded protein precipitates. The protein is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin has not been defined.
Additional Names
CLU, AAG4, APO-J, APOJ, CLI, KUB1, MGC24903, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, apoj, apolipoprotein j, clusterin, complement lysis inhibitor, complement-associated protein sp-40, sgp2, sulfated glycoprotein 2, testosterone-repressed prostate message 2, trpm2
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Description
Left: Immunohistochemistry staining of Clusterin in testis (formalin-fixed paraffin-embedded) using Clusterin monoclonal antibody.
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Purification
Protein A Column
Clonality / Clone
This is a monoclonal antibody. Clone Hs-3.
Isotype
IgG1
Host
Clusterin monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Clusterin monoclonal antibody was raised against freshly ejaculated human sperms washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. (Human)
Application
Clusterin antibody can be used for detection of Clusterin by ICC, ELISA, IHC-parafin (10 μg/ml).
Tested Application
E, IHC, ICC
Buffer
Clusterin monoclonal antibody is supplied in PBS, 15 mM sodium azide, approx., pH 7.4.
Storage
Clusterin monoclonal antibody can be stored at 4˚C for short term storage, for long term storage aliquot and store at -20˚C . Avoid freeze thaw cycles.
Species Reactivity
H
Formulation
Weight: 50 μg
Concentration: 1 mg/ml
Short Description
Clusterin
This product belongs to the following categories:
Datasheet 09-01W
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