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Peptides
ChAT Peptide
Background
ChAT (C-Terminus) Peptide
Please note that this peptide has a delivery time of approximately 3 weeks. If the peptide is out of stock, the delivery time may take up to 1-3 months.
Location
ChAT peptide corresponds to 14 amino acids near the C-terminus region of ChAT.
Preparation
ChAT peptide is supplied as 100μg of dried peptide. When the peptide is reconstituted in 200μl water, the concentration will be 0.5mg/ml.
Application
ChAT peptide is used for blocking the activity of ChAT antibody (catalog number 45-038). The ChAT antibody ships at 0.5mg/ml and the ChAT peptide ships as a 100μl pellet. When peptide is reconstituted in 200μl water, the concentration will be 0.5mg/ml, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
Tested Application
Bl
Buffer
Dried peptide.
Storage
Store at -20˚C. Minimize freezing and thawing.
Species Reactivity
n/a
Protein Accession Number
NP_065574, NP_066264, NP_066265, NP_066266
Short Description
(CT) ChAT peptide
This product belongs to the following categories:
Related Products
Datasheet 0901-W
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