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Primary Antibodies
ChAT Antibody
Background
ChAT Antibody. This antibody is expected to recognize both reported isoforms. NP_066264, NP_066265 and NP_066266 are identical proteins representing isoform 1.
Additional Names
ChAT, CMS1A, CMS1A2, choline acetyltransferase, acetyl CoA:choline O-acetyltransferase
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Description
Left: Western blot analysis of ChAT in human placenta lysate (35μg protein in RIPA buffer) using ChAT antibody (0.03μg/mL).
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Source
ChAT antibody was raised against a synthetic peptide near the C-terminus of ChAT.
Purification
ChAT antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ChAT antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approx 70kDa band observed in human placenta lysates (calculated MW of 70.5kDa according to NP_065574 and NP_066264). Recommended concentration: 0.03-0.3μg/ml.
Tested Application
E, WB
Buffer
ChAT antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-038P - ChAT Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1309 - Human Placenta Lysate
Species Reactivity
H, P, D, Chm
Protein Accession Number
NP_065574, NP_066264, NP_066265, NP_066266
Short Description
(CT) Choline acetyltransferase
This product belongs to the following categories:
Related Products
References- Madziar B, Lopez-Coviella I, Zemelko V, Berse B. Regulation of cholinergic gene expression by nerve growth factor depends on the phosphatidylinositol-3'-kinase pathway. J Neurochem. 2005 Feb;92(4):767-79.
Datasheet 08-02W
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