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Primary Antibodies
ChAT Antibody
Background
ChAT Antibody. This antibody is NOT expected to cross-react to Human CHAT.
Additional Names
ChAT, choline acetyltransferase , B230380D24Rik
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Description
Left: Western blot analysis of ChAT in mouse brain lysate (35μg protein in RIPA buffer) using ChAT antibody (0.2μg/mL).
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Source
ChAT antibody was raised against a synthetic peptide near the C-terminus of ChAT.
Purification
ChAT antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ChAT antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approx 75-80kDa band observed in mouse and rat brain lysates (calculated MW of 72.3kDa according to NP_034021.1). Recommended concentration: 0.2-0.5μg/ml.
Tested Application
E, WB
Buffer
ChAT antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-037P - ChAT Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1403 - Mouse Brain Tissue Lysate
Species Reactivity
M, R
Protein Accession Number
NP_034021.1
Short Description
(CT) ChAT (mouse)
This product belongs to the following categories:
Related Products
References- Contestabile A, Fila T, Bartesaghi R, Contestabile A, Ciani E. Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases. J Neurochem. 2006 Apr;97(2):515-26. Epub 2006 Mar 15.
Datasheet 08-02W
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