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Primary Antibodies
Caspase-9 Antibody
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (3). Caspase-9 is also activated by granzyme B and CPP32 (1,2). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD (4). Caspase-9 is expressed in a variety of human tissues (1,2).
Additional Names
Caspase-9 (IN1), Mch6/ Apaf-3, Casp-9
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Description
Left: Western blot analysis of caspase-9 in HeLa whole cell lysate with caspase-9 antibody at 1:1000 dilution.
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Source
Caspase-9 antibody was raised against a peptide corresponding to amino acids 41 to 56 of human caspase-9 .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Caspase-9 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Caspase-9 (Intermediate Domain 1) Peptide (Cat. No. 2071P)
Application
Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1:1000 to 1:2000 dilution. A 46 kDa band should be detected. It has no cross response to other members in caspase family.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2071P - Caspase-9 Peptide
Storage
Caspase-9 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
28558771
Protein Accession Number
P55211
Short Description
(IN1) A new caspase
This product belongs to the following categories:
Related Products
References- Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol Chem 1996;271:16720-4
- Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1β converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2α are substrates for the apoptotic mediator CPP32. J Biol Chem 1996;271:27099-106
- Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89
- Pan G, O'Rourke K, Dixit VM. Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex. J Biol Chem 1998;273:5841-5 (RD1299)
Datasheet 08-02W
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