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Primary Antibodies
Caspase-5 Antibody
Background
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18 (2,3). Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β (4). Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-ϒ (5).
Additional Names
Caspase-5 (IN), ICH-3 , ICE rel III
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Description
Left: Western blot analysis of caspase-5 in Ramos cells with caspase-5 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunocytochemistry of caspase-5 in P815 cells with caspase-5 antibody at 2 μg/ml.
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Other Product Images
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Source
Caspase-5 antibody was raised against a 14 amino acid peptide from the center of human Caspase-5.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Caspase-5 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Casp-5 / (ICH-3 / ICE rel III (Intermediate Domain) Peptide (Cat. No. 3457P)
Application
Casp-5 antibody can be used for the detection of caspase-5 by Western blot at 0.5 - 1 μg/mlDepending on cell lines or tissues used, other cleavage products may be observed.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3457P - Casp-5 Peptide
Storage
Caspase-5 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1225 - Ramos Cell Lysate
- Cat. No. 1286 - P815 Cell Lysate
Species Reactivity
H
Protein GI Number
212276423
Protein Accession Number
P51878
Short Description
(IN) a caspase in the inflammatory subfamily
This product belongs to the following categories:
Related Products
References- Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
- Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
- Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
- Martinon F, Burns K, and Tschopp J. The inflammasome: a molecular platform triggering activation of the inflammatory caspases and processing of proIL-1?. Mol. Cell. 2002; 10:417-26.
Datasheet 08-02W
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