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Primary Antibodies
Caspase-4 Antibody
Background
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18 (2,3). Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells (4). More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer’s disease (5).
Additional Names
Caspase-4 (NT), ICH-2, ICE rel II , Mih1
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Description
Left: Western blot analysis of caspase-4 in Ramos cells with caspase-4 antibody at (A) 0.5 and (B) 1 μgg/ml.
Below: Immunohistochemical staining of mouse spleen using caspase-4 antibody at 2 μg/ml.
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Other Product Images
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Source
Caspase-4 antibody was raised against a 16 amino acid peptide from the amino-terminus of human Caspase-4.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Caspase-4 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Casp-4 / ICH-2 / ICE rel II / Mih1 (N-Terminus) Peptide (Cat. No. 3451P)
Application
Casp-4 antibody can be used for the detection of Caspase-4 by Western blot at 1 μg/ml. Depending on cell lines or tissues used, other cleavage products may be observed.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3451P - Casp-4 Peptide
Storage
Caspase-4 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1225 - Ramos Cell Lysate
- Cat. No. 1406 - Mouse Spleen Tissue Lysate
Species Reactivity
H, M
Protein GI Number
886050
Protein Accession Number
AAA86890
Short Description
(NT) a caspase induced by ER stress
This product belongs to the following categories:
Related Products
References- Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
- Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
- Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
- Kamens J, Paskind M, Hugunin M, et al. Identification and characterization of ICH-2, a novel member of the interleukin-1 β-converting enzyme family of cysteine proteases. J. Biol. Chem. 1995; 270:15250-6.
Datasheet 08-02W
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