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Primary Antibodies
Caspase-2 Antibody
Background
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18 (3,4). Although phylogenetically similar to this subfamily, Caspase-2 is thought to be involved in stress-induced apoptosis (5). Caspase-2 has two major isoforms; overexpression on the long form results in apoptosis while that of the short form suppresses cell death (6).
Additional Names
Caspase-2 (NT), ICH-1, Nedd2
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Description
Left: Western blot analysis of caspase-2 in Ramos cells with caspase-2 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunocytochemistry of caspase-2 in A-20 cells with caspase-2 antibody at 2 μg/ml.
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Other Product Images
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Source
Caspase-2 antibody was raised against a 16 amino acid peptide from the amino-terminus of human Caspase-2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Caspase-2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Murine Casp-2 / ICH-1 / Nedd2 (N-Terminus) Peptide (Cat. No. 3447P)
Application
Caspase-2 antibody can be used for the detection of caspase-2 by Western blot at 1 μg/ml. Depending on cell lines or tissues used, other cleavage products may be observed.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3447P - Casp-2 Peptide
Storage
Caspase-2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1225 - Ramos Cell Lysate
- Cat. No. 1288 - A20 Cell Lysate
Species Reactivity
H, M
Protein GI Number
83300977
Protein Accession Number
P42575
Short Description
(NT) a caspase induced by ER stress and DNA damage
This product belongs to the following categories:
Related Products
References- Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
- Zhivotovsky B and Orrenius S. Caspase-2 funtion in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
- Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
- Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
Datasheet 08-02W
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