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Primary Antibodies
Caspase-13 Antibody
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death is finally caused by members of the caspase family of proteases and caspase activated DNases. A novel member in the caspase family was recently identified and designated ERICE (for Evolutionarily Related Interleukin-1 b Converting Enzyme) and caspase-13?(1). Caspase-13 belongs to the ICE subfamily of caspases. Overexpression of caspase-13 induces apoptosis. Caspase-13 was activated by caspase-8, which is a key enzyme in death receptor induced apoptosis. Caspase-13 is expressed in a variety of human tissues and cell lines (1).
Additional Names
Caspase-13, Casp-13, ERICE
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Description
Left: Western blot analysis of caspase-13 in human HL60 cell lysate (A), mouse brain (B) and rat brain (C) tissue lysates with caspase-13 antibody at 1 μg/ml.
Below: Immunohistochemistry of caspase-13 in mouse brain tissue with caspase-13 antibody at 10 μg/ml.
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Other Product Images
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Source
Caspase-13 antibody was raised against a peptide corresponding to amino acids 91 to 105 of human caspase-13.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
Caspase-13 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Caspase-13 Peptide (Cat. No. 2245P)
Application
Caspase-13 antibody can be used for detection of caspase-13 by Western blot at 0.5 to 1 μg/ml. A 43 kDa band should be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2245P - Caspase-13 Peptide
Storage
Caspase-13 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1209 - HL60 Cell Lysate
- Cat. No. 1403 - Mouse Brain Tissue Lysate
- Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
10720034
Protein Accession Number
O75601
Short Description
Evolutionarily Related ICE
This product belongs to the following categories:
Related Products
References- Humke EW, Ni J, Dixit VM. ERICE, a novel FLICE-activatable caspase. J Biol Chem 1998;273:15702-7 (WD0500)
Datasheet 08-02W
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